'Separation' in the Bible

Then Jesus said, “I came into this world for judgment [to separate those who believe in Me from those who reject Me—to declare judgment on those who choose to be separated from God], so that the sightless would see, and those who see would become blind.”

Yet if the unbeliever departs, let there be separation. The brother or the sister is not under bondage in such cases, but God has called us in peace.

But [when] we were made orphans by separation from you, brothers, for {a short time} (in face, not in heart), [we were] even more eager with great desire to see your face,

Therefore, when we could no longer endure our separation [from you], we thought it best to be left behind, alone at Athens,

In the days of His earthly life, Jesus offered up both [specific] petitions and [urgent] supplications [for that which He needed] with fervent crying and tears to the One who was [always] able to save Him from death, and He was heard because of His reverent submission toward God [His sinlessness and His unfailing determination to do the Father’s will].

for they, indeed, for a few days, according to what seemed good to them, were chastening, but He for profit, to be partakers of His separation;

peace pursue with all, and the separation, apart from which no one shall see the Lord,